SBV - Schmallenberg Virus PCR detection kit


Test for the detection of Schmallenberg virus by enzymatic gene amplification

SBV - Schmallenberg Virus PCR detection kit

ADIAVETTM Schmallenberg Virus

Ready to use kit
Single-well duplex kit
Validated by French Reference Laboratory (ANSES Maisons-Alfort)
12 months shelflife
 
Pathogen(s)
SBV - Schmallenberg virus
Target(s)
L segment
Internal control
Endogenous (GAPDH), validates extraction and amplification
Technology
Real Time PCR
Dyes
FAM (SBV)
VIC (Internal control)
Storage
-20°C
Sample(s)
Tissues (brain, spleen), whole blood, serum
Specie(s)
Bovine, ovine, caprine
Extraction of nucleic acid
Silica column, magnetic beads
 
Reactions / Kit
Product number
50 reactions
ADI491-50
100 reactions
ADI491-100
 





































Pathology & diagnosis

The Schmallenberg virus was isolated for the first time in Germany in 2011 by Friedrich-Loeffler-Institut (FLI) from blood of infected cows. The name is based on the geographic origin of the virus (village of the North Rhine-Westphalia). First phylogenic analyses show that the viral genome presents the most similar sequences with Shamonda viruses within the Simbu serogroup. These suggest that the novel virus is a Shamonda-like virus within the genus Orthobynyavirus.

Clinical signs of Schmallenberg virus infection in adult ruminants are mainly mild or non-existent but transient fever, loss of appetite, a reduction in milk yield and diarrhoea have been observed. The main clinical signs of Schmallenberg virus are congenital malformations (severe arthrogryposis, torticollis, brachygnathia, hydrocephalus and other severe brain malformations) in newborn animals similar to those observed in infections by the Akabane virus, the main known virus of the genus.

If infection occurs prior to pregnancy a normal pregnancy is expected to occur. Malformations in foetuses would be observed when the infection occurs during a vulnerable stage of the pregnancy. In analogy to Akabane virus, the vulnerable stage of pregnancy may be between days 28 and 36 in sheep and between days 75 and 110 in cattle.

The viruses of Simbu serogroup are transmitted by insects (Culicoides midges and mosquitoes). It is likely that Schmallenberg virus is also transmitted by these insects but this has not been confirmed yet. This way of transmission is reinforced by the clinical signs of Schmallenberg virus infection in adults that were observed from August onwards, coinciding with the density peak of the putative vectors. Considering a gestation period of 5 and 9 months for respectively sheep/goats and cows it could be expected that the majority of the deformed lambs/kids would be born from December to February and the majority of deformed calves between March and May.

Today, neither vaccine nor treatment exists against the viral infection and no serological test is available. Viral culture and PCR amplification are the only ways to detect the virus.

Current knowledge suggests that it is unlikely that Schmallenberg virus can cause disease in humans.
 

ADIAVETTM PCR products are now part of a global diagnostic offer inside the veterinary franchise of bioMérieux Industry. Find out more on ADIAVET webpage